KMID : 1377020230200010111
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Tissue Engineering and Regenerative Medicine 2023 Volume.20 No. 1 p.111 ~ p.125
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Laser-Structured Si and PLGA Inhibit the Neuro2a Differentiation in Mono- and Co-Culture with Glia
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Angelaki Despoina
Kavatzikidou Paraskevi Fotakis Costas Stratakis Emmanuel Ranella Anthi
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Abstract
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Background: The first step towards a successful neural tissue engineering therapy is the development of an appropriate scaffold and the in vitro study of the cellular response onto it.
Methods: Here, we fabricated nano- and micro- patterned Si surfaces via direct ultrafast laser irradiation, as well as their replicas in the biodegradable poly(lactide-co-glycolide), in order to use them as culture substrates for neuronal cells. The differentiation of neuro2a cells on the Si platforms and their replicas was studied both in a mono-culture and in a co-culture with glial cells (Schwann?SW10).
Results: It was found that the substrate¡¯s roughness inhibits the differentiation of the neuronal cells even in the presence of the differentiation medium, and the higher the roughness is, the more the differentiation gets limited.
Conclusion: Our results highlight the importance of the substrate¡¯s topography for the controlled growth and differentiation of the neuronal cells and their further study via protein screening methods could shed light on the factors that lead to limited differentiation; thus, contributing to the long standing request for culture substrates that induce cells to differentiate.
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KEYWORD
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Neuronal differentiation, Glia-neurons co-culture, Nano/micro topography, Laser structuring, Neural tissue engineering
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